BacT/Alert and Isolator Blood Culture Systems

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False-negative thermonuclease tests with new BacTAlert blood culture bottles.

The Microbiology Unit of Southern Cross Pathology Australia, Monash Medical Centre, has used the Organon Technika BacTAlert automated blood culture instrument since 1995 for the detection of causative organisms of septicemia and bacteremia. When a blood culture bottle is flagged as positive, it is removed from the instrument and subculturing and Gram staining are performed. Gram-positive cocci,...

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Clinical laboratory comparison of the 10-ml isolator blood culture system with BACTEC radiometric blood culture media.

The efficiency of the 10-ml Isolator (E. I. du Pont de Nemours & Co., Inc.) for recovery of pathogens from blood was compared with that of BACTEC 6B and 7C media (Johnston Laboratories) by using 4,195 cultures from 1,662 patients. During the first phase of the study, BACTEC bottles were inoculated with 3 ml of blood; in the second phase, bottles were inoculated with 5 ml. The objectives were to...

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Controlled clinical comparison of two lysis-based blood culture systems, isolator and Septi-Chek Release, for detection of bloodstream infections.

A controlled clinical comparison was made of the Isolator (Wampole Laboratories, Cranbury, N.J.) and the Septi-Chek Release bottle (Roche Diagnostics, Nutley, N.J.). From 6,345 blood culture sets fulfilling minimum criteria for volume of blood cultured, 840 strains were isolated, of which only 691 (82%) were considered to be representative of bloodstream infection according to Centers for Disea...

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Clinical comparison of isolator and BACTEC 660 resin media for blood culture.

The 10-ml Isolator system (E.I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC 16A-17A nonradiometric resin system (Johnston Laboratories, Inc., Towson, Md.) for isolation of organisms from 6,839 paired blood cultures. Equal volumes of blood (6 to 10 ml for each Isolator and 3 to 5 ml for each BACTEC bottle) were cultured in parallel in the two systems, and 600 i...

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Mycobacterium tuberculosis bacteremia detected by the Isolator lysis-centrifugation blood culture system.

Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional laboratory media inoculated with pleural fluid from the patient.

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ژورنال

عنوان ژورنال: American Journal of Clinical Pathology

سال: 1997

ISSN: 0002-9173,1943-7722

DOI: 10.1093/ajcp/107.3.376